| 摘要: |
| 目的 建立一种测定啤酒易拉罐中苯酚和双酚A迁移量的方法，应用于市售易拉罐啤酒污染物的检测。方法 选用体积分数为10%的乙醇为食品模拟物，在迁移温度为60 ℃，迁移时间为10 d的条件下开展迁移研究，采用高效液相色谱-荧光法对迁移样液进行检测。结果 在文中的试验方法下，苯酚和双酚A在7 min内可实现完全分离，方法的检出限（LOD）分别为6.0和1.5 μg/L。苯酚在20~400 μg/L质量浓度范围内线性良好（相关系数为0.9999），双酚A在5~100 μg/L质量浓度范围内线性良好（相关系数为0.9998）。通过对空白样品进行加标回收试验，苯酚回收率为90.0%~98.5%，相对标准偏差（RSD）为2.7%~6.2%；双酚A回收率为84.0%~102%，RSD为3.2%~5.3%。将该方法应用于7种易拉罐啤酒中苯酚和双酚A迁移量的检测，结果均未检出苯酚，双酚A检出量在6.9~14.8 μg/kg之间。结论 该方法准确度高、灵敏度好，能够满足啤酒易拉罐中苯酚和双酚A迁移量检测的要求。 |
| 关键词: 苯酚 双酚A 啤酒易拉罐 迁移量 高效液相色谱法 |
| DOI：10.19554/j.cnki.1001-3563.2018.09.016 |
| 分类号:TB489；TS201.6 |
| 基金项目:国家自然科学基金（21706222） |
|
| Detection of Phenol and Bisphenol A Migration in Beer Cans |
|
BAI Ze-qing
|
|
Xiamen Products Quality Supervision & Inspection Institute, Xiamen 361005, China
|
| Abstract: |
| The work aims to establish a new method for the determination of the migration amounts of phenol and bisphenol A (BPA) in beer cans and apply such method in the contaminant detection of commercially available canned beer. The ethyl alcohol (volume fraction: 10%) was selected as the food simulant to carry out migration studies when the migration temperature was at 60 ℃ and the migration time was 10 days. The migration sample solution was detected by high performance liquid chromatography-fluorescence. In the proposed method, phenol and BPA were separated completely in 7 min. The limit of detection (LOD) of the method was 6.0 μg/L for phenol and 1.5 μg/L for BPA. The correlation coefficient in the range of 20~400 μg/L (mass concentration) was 0.9999 for phenol and 0.9998 in the range of 5~100 μg/L (mass concentration) for BPA. The spiked recovery of phenol was 90.0%~98.5% with RSDs ranging from 2.7% to 6.2% while that was 84.0%~102% with RSDs ranging from 3.2% to 5.3% for BPA. The method was further applied to the migration detection of phenol and BPA in 7 kinds of canned beers. Phenol was not detectable, while the detected migration amounts of BPA varied in the range of 6.9~14.8 μg/kg. With high accuracy and good sensitivity, the method can meet the detection demands of phenol and BPA migration amounts in beer cans. |
| Key words: phenol bisphenol A beer cans migration amount high performance liquid chromatography |
渝公网安备 50010702501716号